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Determining the relative amounts of known and unknown metabolites in cells, tissues and biological fluids

Harvard Medical School

Service Description

Metabolic Phenotyping Core

Providing users with access to high-quality model creation and detailed metabolic phenotyping.

  • Metabolomics (~400 aqueous/lipid metabolites)
  • GC-MS fatty acid profiling/chromatography

Have Questions? Contact Us.

Alexander Soukas, MD, PhD, Core Director • Email 

Robert Gerszten, Co-Director • Email

University of Michigan

Service Description

Molecular Phenotyping Core

The Directed Metabolomics Core Lab provides the  research community with the ability to perform quantitative assays for a wide variety of small molecules of biological origin (metabolites). The lab works with individual investigators to develop appropriate methods for metabolite analysis. We work with a wide range of sample types, including (but not limited to) cells, tissues, and biological fluids from human subjects, research animals, or other organisms. Typical assays we perform are listed below; however, our capabilities are by no means limited to the compound classes in the list. Please contact us with any inquiries about your analysis of interest.

Amino Acid Analysis

  • GC-MS total ion chromatogram for derivatized amino acids in human plasma

Glycolysis /TCA Cycle /Nucleotide Analysis

  • LC-MS extracted ion chromatograms for selected TCA intermediates in human plasma

Acyl-Carnitine Analysis

  • LC-MS extracted ion chromatograms for acyl-carnitines in human plasma

Have Questions? Contact Us.

Maureen Kachman, PhD, Managing Director
Email • Phone: (734) 232-0842

Service Description

Molecular Phenotyping Core

The Lipidomics Lab provides interested researchers an opportunity to quantify and identify lipids and other biological material in cells, organs or other biological fluids of test animals or in human subjects. The lab works with individual investigators to develop directed methods for metabolite analysis. The analytic platforms include mass spectrometry instrumentation in several configurations (GC/MS, LC/MS, MS/MS) and NMR-based mass isotopomer analysis for measurement of flux in specific metabolic pathways.

Have Questions? Contact Us.

Maureen Kachman, PhD, Managing Director
Email • Phone: (734) 232-0842

Service Description

Molecular Phenotyping Core

The Undirected Metabolomics Lab is firmly committed to the development of the science of Metabolomics, and discovery of advanced metabolomic techniques. In order to accomplish this goal, we freely collaborate with researchers from all over the world to acquire a diverse collection of sample types and experimental designs.

The Undirected Metabolomics Facility platform is based on four interwoven and fully integrated aspects:

Hardware. Because of its high sensitivity and the specificity of high-resolution mass accuracy, our platforms are Mass spectrally-based. The current generation of mass specs and the software that control them have become so powerful that it is likely that mass spectrally-based platforms will be the workhorses of the science for the foreseeable future, and can solve the major problems. None-the-less, NMR, FTIR, and other physical measurement systems clearly add benefit where mass spec is not sufficient.

Informatics. The science of Metabolomics is far more driven by the required informatics infrastructure than by the actual hardware platform. The informatics aspect begins with a LIMS system capable of tracking samples, their process through the analysis, and the results of their analysis.

Robotics. Humans are too good at thinking and being creative to do the repetitive mundane, and error prone tasks involved in most medium through-put sample handling. By automating processes wherever possible, we are able to reduce error and, thereby, enhance data quality.

Standards-Based QA/QC. The use of standards, embedded into all samples and blanks, to continuously monitor all aspects of the sample preparation and analytical processes assures that the overall process may be continually improved. In addition, the ability to always know the level of error in any data set it is possible to design better experiments. Better experimental design yields more better quality information is obtainable from fewer samples.

Have Questions? Contact Us.

Maureen Kachman, PhD, Managing Director
Email • Phone: (734) 232-0842

University of North Carolina at Chapel Hill

Service Description

Metabolism and Metabolomics Core

Metabolomics, the profiling of the many small molecules in blood, urine or tissues, provides an opportunity for investigators to obtain a snapshot of metabolism. The metabolomics lab, located in Kannapolis, NC, is working to make personalized medicine a reality through metabolomics. Metabolomic analyses provide a more comprehensive view of a patient’s metabolism than the limited measurements of glucose and cholesterol that doctors employ today. Using metabolomics, the lab assesses differences in the metabolic profile of individuals that correlate with states of wellness or disease.

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Susan Sumner, PhD, Core Director
Email • (704) 250-5066

Susan McRitchie, MA, MS, Program Manager
Email • (704) 250-5067