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Examining the effects of dietary intake on health-related outcomes in humans

New York

Service Description

Human Phenotyping Core

Specialized measurements for adipose tissue analysis in human subjects.

  • Isolation of primary adipocytes and stromal vascular fraction
  • Quantification and isolation of adipose tissue immune cells (macrophages, lymphocytes and NK cells) by Fluorescence Activated Cell Sorting (FACS)
  • Quantitative PCR for adipokine gene expression in whole fat, adipocytes, stromal vascular fraction, and macrophages
  • Western blot analysis for insulin signal transduction elements
  • Quantitative assessment of macrophages infiltration by immunostaining
  • Determination of adipocyte size and number in paraffin-embedded samples by computer-assisted analysis
  • Adipose tissue lipoprotein lipase activity in frozen samples
  • In vitro adipose tissue lipolysis and glucose uptake
  • Culture and differentiation of primary preadipocytes in vitro
  • Adipokine secretion assay
  • Adipose tissue histology
    • Machine processing and embedding
    • Paraffin tissue sectioning
    • Immunohistochemistry
    • H&E staining
  • Training in adipose tissue sample preparation and characterization
    • Protein extraction
    • RNA extraction
    • Adipose tissue fixation
    • Adipose tissue morphologic analysis

Have Questions? Contact Us.

Yiying Zhang, PhD, Sub-Core Director
Email • Phone: (212) 851-5301

Maryland, Mid-Atlantic

Service Description

Clinical and Translational Research Core

The Clinical and Translational Research Core provides  assistance in clinical research relating to obesity, nutrition and physical activity, including support for genotype-driven recruitment.

Comprehensive, state-of-the-art clinical evaluations to characterize (phenotype) subjects including:

  • Body composition (DXA, CT, MRI, BodPod: total body fat, lean mass, visceral, hepatic and intramuscular fat, bone density)
  • Patient registry
  • Dietary assessment, physical activity habits
  • VO2max, function, mobility, accelerometry
  • Lipids, glucose tolerance, inflammatory cytokines

Clinical, endocrine-metabolic and nutrition assays (e.g., glucose, insulin, OGTT, lipids, cytokines)

  • Insulin sensitivity, cytokines, hormones and metabolites, bone-mineral metabolism (University of Maryland at Baltimore)
  • Muscle metabolism, insulin action, mitochondrial function, enzyme activity and substrate assays (University of Maryland at Baltimore)
  • Cardiovascular function (brachial artery reactivity, blood flow), endogenous fibrinolysis (University of Maryland at Baltimore)
  • Energy expenditure
  • Adipose tissue aspirations and processing
  • Muscle biopsy and other tissues (liver, gastric, jejunum, fecal) processing,  specimen processing, storage, shipment

Exercise and nutritional intervention

  • Dietary and calorie restriction-weight loss, exercise, and pharmacological treatments (University of Maryland at Baltimore, Johns Hopkins University)
  • Use of specialized resources, such as a metabolic kitchen for meal preparation, dining room, nutrient assessment services, exercise testing and training facilities

Have Questions? Contact Us.

Alice Ryan, PhD, Core Co-Director
Email • Phone: (410) 605-7851

Pennington Biomedical Research Center

Service Description

Human Phenotyping Core (Behavioral Subcore)

Visual Analogue Scales are available to quantify subjective ratings of appetite. Taste preference, which is associated with body weight, can be assessed with the Drewnowski taste test, and taste sensitivity can be quantified using bitter compounds. The liking and wanting procedure is available to assess explicit liking and wanting, followed by implicit wanting for the same target food stimuli.

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Corby K. Martin, PhD, Core Director
Email • Phone: (225) 763-2585

Service Description

Human Phenotyping Core (Behavioral Subcore)

Behavior change and dietary interventions services are available to users who require behavioral, psychological, or dietary expertise and consultation. Using theories of behavioral change and the expertise of registered dieticians and psychologists, interventions are developed and deployed to meet the demands of the study protocol. Interventions may be offered for weight loss, weight maintenance, weight gain (as in gestational studies).All topics are designed by those with expertise in dietary and psychological principles.

Have Questions? Contact Us.

Corby K. Martin, PhD, Core Director
Email • Phone: (225) 763-2585

Service Description

Human Phenotyping Core (Behavioral Subcore)

Services are available to: 1) quantify food intake among participants, and 2) test the effect of interventions on eating behavior. Food intake is quantified by covertly weighing food provision and plate waste or with one of two Universal Eating Monitors (UEM), which consist of a scale concealed in a table that is connected to a computer that continuously records food intake.

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Corby K. Martin, PhD, Core Director
Email • Phone: (225) 763-2585

Service Description

Human Phenotyping Core (Behavioral Subcore)

Pennington/Louisiana NORC investigators and collaborators adapted the Remote Food Photography Method© and SmartIntake® app to assess the food intake of the following populations:

  • Bottle Fed Infants
  • Children
  • Adolescents
  • Pregnant Women

Have Questions? Contact Us.

Corby K. Martin, PhD, Core Director
Email • Phone: (225) 763-2585

Service Description

Human Phenotyping Core (Behavioral Subcore)

Food intake can be quantified while people live in their natural environment using the Remote Food Photography Method© (RFPM) and the SmartIntake® smartphone app, which were developed by Pennington/Louisiana NORC investigators.

Have Questions? Contact Us.

Corby K. Martin, PhD, Core Director
Email • Phone: (225) 763-2585

University of Michigan

Service Description

Human Phenotyping Core (Nutrient Assessment Lab)

Nutrition Counseling

  • Specific or general dietary counseling provided to participants as part of a study protocol

Specialized Consulting

  • Available to study teams looking for advanced guidance in designing and implementing nutrition interventions/assessments or analyzing dietary data

24-Hour Recalls

  • Administration, data collection and processing utilizing the Nutrition Data System for Research (NDSR)

Food Records & Diaries

  • Participant instruction, data collection and processing using NDSR

Meal Preparation

  • Meals involving specialized preparation (weighing and measuring of foods to fit specific needs), no charge for pre-prepared hospital items

Have Questions? Contact Us.

Human Phenotyping Core •  Email 

 

University of North Carolina at Chapel Hill

unc_adipose-tissue-biopsy
Adipose Tissue Biospy

Service Description

Human Research Core (CHAI Core: Clinical Assessment)

Fat pad aspiration to obtain Adipose Tissue samples is performed using an 18 G 1 ½ inch needle technique. Local anesthesia is applied to diminish discomfort of the subject. The technique is minimally invasive with a very low rate for complications or post procedure infection. Adipose tissue collected is preserved according to the study specifications and handed to the primary investigator to be processed by their staff.

Have Questions? Contact Us.

Martin Kohlmeier, MD, PhD, Core Director
Email • Phone: (704) 250-5035

 

 

 

 

 

BodPod
BodPod
PeaPod
PeaPod

Service Description

Human Research Core (CHAI Core: Clinical Assessment)

The BodPod is the gold standard in body composition assessment, with the ability to analyze across the lifespan. The BodPod is fast, accurate, non-invasive and accommodates a variety of populations. This equipment uses air displacement technology to determine body volume and density. The PeaPod pediatric option accessory is also available for use with the BodPod to accommodate children 2-6 years of age. The PeaPod measures the same variables in infants up to 6 months.

Have Questions? Contact Us.

Martin Kohlmeier, MD, PhD, Core Director
Email • Phone: (704) 250-5035

 

 

 

Computer-Assisted Sperm Analysis
Computer-Assisted Sperm Analysis

Service Description

Human Research Core (CHAI Core: Clinical Assessment)

Computer Assisted Sperm Analysis provides an automated and accurate examination of sperm concentration and motility. Reduced bias is compared with visual evaluation and software is available to assess human and animal samples.

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Martin Kohlmeier, MD, PhD, Core Director
Email • Phone: (704) 250-5035

 

 

 

Diet Assessment
Diet Assessment

Service Description

Diet and Physical Activity Assessment Core (CHAI Core: Behavioral Assessment)

Expertise is provided for all stages of Diet Assessment from development and data collection to data analysis. Data collection is done using a variety of high end and validated methods at a competitive rate. Highly skilled and experienced, our research team will collect your dietary data in both English and Spanish. All diet assessments are a turn-key operation where deliverables include data, protocol documentation, and data file descriptions.

Data collection is also provided for participants of all ages, with a special emphasis on pediatric dietary assessment. We offer expert advice for international diet data collection and current methods to statistically model and account for measurement errors in dietary data.

  • Consultation and grant development for measurement issues related to diet and physical activity
  • Diet assessment measurement
  • Collect, analyze and process diet data
  • Provide trained and experienced research staff
  • Able to collect diet information in Spanish and English
  • Employ top of the line diet software
  • Nutrition Data System for Research (24-hour diet recalls, food record data entry, menu/user recipe analysis)
  • Custom food frequency questionnaires-development, implementation, and analysis

Have Questions? Contact Us.

Kimberly P. Truesdale, PhD, Core Director
Email • Phone: (919) 966-7237

 

 

Dynamometer
Dynamometer

Service Description

Human Research Core (CHAI Core: Clinical Assessment)

The Dynamometer (BIODEX) provides testing and rehabilitation services for the knee, ankle, and hips plus the shoulder, elbow, forearm, and wrist. A variety of output reports allow numeric and graphic information to be printed in a number of different formats. Isokinetic testing can be used to provide a valid, reliable, objective measure of a muscle’s performance time after time.

Have Questions? Contact Us.

Martin Kohlmeier, MD, PhD, Core Director
Email • Phone: (704) 250-5035

 

 

 

Service Description

Human Research Core (CHAI Core: Clinical Assessment)

Using Transient Elastography, the Fibroscan technology assists with the assessment, diagnosis, and disease progression/regression evaluation of fatty liver disease and fibrosis. This technology is non-invasive, painless, and provides a fast evaluation procedure that allows for more frequent serial testing providing longitudinal data of the disease when required.

Have Questions? Contact Us.

Martin Kohlmeier, MD, PhD, Core Director
Email • Phone: (704) 250-5035

 

 

Hematology Analyzer
Hematology Analyzer

Service Description

Human Research Core (CHAI Core: Clinical Assessment)

The Hematology Analyzer offers a 5-part differential utilizing a closed tube sampling system to deliver consistent results with a fast turnaround time.  This windows based operating system is able to accommodate pediatric samples.

Have Questions? Contact Us.

Martin Kohlmeier, MD, PhD, Core Director
Email • Phone: (704) 250-5035

 

 

Metabolic Cart
Metabolic Cart

Service Description

Human Research Core (CHAI Core: Clinical Assessment)

Metabolic Carts are available to measure resting metabolic rate and exercise induced changes in energy expenditure. Testing can utilize a treadmill (Quinton Q-Stress System) or stationary bike (Lode Corival Bike Ergometer).

Have Questions? Contact Us.

Martin Kohlmeier, MD, PhD, Core Director
Email • Phone: (704) 250-5035

 

 

Metabolic Research Kitchen
Metabolic Research Kitchen

Service Description

Human Research Core (CHAI Core: Clinical Assessment)

This Metabolic Research Kitchen is used to prepare and deliver meals of exact composition. Investigators work with a Registered Dietitian to design, prepare and serve meals with a safe, accurate and consistent approach. The Kitchen is equipped with commercial appliances and professional software. A spacious dining area, adjacent to the kitchen, is also available to accommodate up to 50 research participants for diet intervention studies.

Have Questions? Contact Us.

Martin Kohlmeier, MD, PhD, Core Director
Email • Phone: (704) 250-5035

 

 

Mobile Body Composition Clinic
Mobile Body Composition Clinic

Service Description

Human Research Core (CHAI Core: Clinical Assessment)

The Mobile Body Composition Clinic is a portable trailer, available for travel in and around the Chapel Hill and Kannapolis areas in North Carolina. It is equipped with the latest technologies to accurately measure body fat and lean body mass. The BodPod is fast, accurate, non-invasive and accommodates a variety of populations. This equipment uses air displacement technology to determine body volume and density. This mobile clinic can be customized for your study needs and can include a metabolic cart, scales, body fat calipers, and measuring tapes.

The clinic is fully equipped as a portable laboratory with office space and air conditioning/heat for comfort and efficiency.

Related News and Publications

Have Questions? Contact Us.

Martin Kohlmeier, MD, PhD, Core Director
Email • Phone: (704) 250-5035

 

 

 

 

Phlebotomy Services
Phlebotomy Services

Service Description

Human Research Core (CHAI Core: Clinical Assessment)

Clinic Phlebotomy Services includes urine and stool collection, pregnancy tests and other laboratory procedures. Processing Phlebotomy Services includes includes centrifuging of blood, urine, and other samples as needed for testing, shipping, or storage.

Have Questions? Contact Us.

Martin Kohlmeier, MD, PhD, Core Director
Email • Phone: (704) 250-5035

 

 

 

 

Quantiferon Gold TB Blood Tests
Quantiferon Gold TB Blood Tests

Service Description

Human Research Core (CHAI Core: Clinical Assessment)

The Quantiferon Gold Tuberculosis (TB) Blood Test is an FDA-approved test that screens for prior exposure to tuberculosis (TB). It requires a single blood draw, it can be used as an alternative to the skin test or in cases that the person has a history of a positive skin test (PPD) to follow up their health status and also can be used as an alternative to yearly chest x-rays. This test can help differentiate between a false positive reading and a false negative skin test. When the Quantiferon Gold blood test result is positive or indeterminate you will likely be required by your Primary Care Provider to get a chest x-ray to confirm that you do not have active tuberculosis. A negative result usually means that no further work up is needed. We will draw your blood and send out to the laboratory for analysis.

Have Questions? Contact Us.

Martin Kohlmeier, MD, PhD, Core Director
Email • Phone: (704) 250-5035

 

 

 

Sample Processin
Sample Processing

Service Description

Human Research Core (CHAI Core: Clinical Assessment)

These special samples are not collected by the Human Research Core staff, but by the investigators. They will be labeled, allocated into the appropriate laboratory collection system, orders will be placed in the system and sent out to the Carolinas Healthcare System (CMC) for processing. Results will be collected and reported to the primary investigator after processing of the samples is completed.

Have Questions? Contact Us.

Martin Kohlmeier, MD, PhD, Core Director
Email • Phone: (704) 250-5035

 

 

Skeletal Muscle Biospy
Skeletal Muscle Biospy

Service Description

Human Research Core (CHAI Core: Clinical Assessment)

Human Skeletal Muscle Biopsy is obtained using the modified Bergstrom Technique. This percutaneous biopsy technique allows for collection of skeletal muscle tissue samples in a safe and highly effective manner from the Vastus Lateralis muscle. Local anesthesia is applied to diminish discomfort of the subject. Post procedure instructions are provided to the subject after the procedure. Tissue adhesive is applied at the incision site avoiding the need for follow up of the subject. Tissue sample are preserved under the specifications of the primary investigator and handed over to their staff for further processing.

Have Questions? Contact Us.

Martin Kohlmeier, MD, PhD, Core Director
Email • Phone: (704) 250-5035

 

 

Whole Room Calorimeter
Whole Room Calorimeter

Service Description

Human Research Core (CHAI Core: Clinical Assessment)

The Whole Room Calorimeter is an advanced research suite that uses indirect calorimetry to evaluate a research participant’s 24-hour energy balance (intake and expenditure). The suite is equipped with a bed, stationary bike, bathroom, airlock chamber for entry of food, ports for blood draws and entertainment options. Data can be collected without interruption during meals, sleep and light activity.

Have Questions? Contact Us.

Martin Kohlmeier, MD, PhD, Core Director
Email • Phone: (704) 250-5035

Washington University, St. Louis

Service Description

Clinical Science Research Core

The Clinical Science Research Core facilitates the performance of complex and diverse clinical research activities through:

  • Subject recruitment
  • Metabolic study design and performance consultation
  • Infusate preparation
  • Muscle and adipose tissue acquisition
  • Intestinal tissue acquisition (equipment use only)
  • Body composition analyses: DEXA, BodPod, PeaPod, MRI, MRS
  • Aerobic fitness testing
  • Strength training and physical performance testing
  • Indirect calorimetry
  • Lifestyle intervention
  • Cardiovascular assessments
  • Arterial and venous catheterization and regional blood flow measurements
  • Ingestive behavior testing
  • Sample processing and GC/MS or GC analysis
  • Kinetic analysis and mathematical modeling of metabolic kinetics
  • Training in any of the above listed services

Have Questions? Contact Us.

Bettina Mittendorfer, Core Director
Email • Phone: (314) 362-8450